丙型肝炎患者肝组织中病毒抗原检测

DETECTION OF HEPATITIS C VIRUS ANTIGENS IN LIVER TISSUES OF PATIENTS WITH HEPATITIS C

目的研究丙型肝炎病毒（ＨＣＶ）抗原在患者肝组织中的分布状况及其相互关系。方法采取免疫组织化学方法，用多克隆抗－ＨＣＶ和单克隆抗－ＨＣＶ－ＮＳ３、抗－ＨＣＶ－ＮＳ５，对１０７例ＨＣＶ感染患者肝组织中ＨＣＶ抗原进行检测。结果应用三种抗体均从ＨＣＶ患者肝组织中检出相应抗原，检出率分别为３８．３％，２８．０％和５２．３％；阳性颗粒定位于肝细胞浆；阳性细胞分布呈散在、弥漫状和簇状；ＨＣＶ－ＮＳ３的表达与炎症反应无明显解剖学关系，ＨＣＶ－ＮＳ５阳性细胞较常见位于炎症灶旁或灶中。结论ＨＣＶ各成分在肝组织中的表达水平存在差别；ＨＣＶ－ＮＳ５的表达可能与ＨＣＶ的致病机制有关。

Objective To investigate distributions and correlation of HCV antigens in the liver tissues. Methods Immunohistochemical techniques such as polyclonal antibody to HCV, monoclonal antibody to HCV-NS3 and monoclonal antibody to HCV-NS5 were used to detect HCV antigens in 107 patients with hepatitis C. Results HCV antigens in liver tissues could be detected by these three kinds of antibodies. The positive rates were 38.3, 28.0 and 52.3%, respectively. The positive granules were located in the cytoplasma of liver cells. The distributions of positive cells were scattered, diffusive and clustered. No obvious anatomic association was observed between the expression of HCV-NS3 and inflammatory reaction. HCV-NS5 positive cells frequently appeared around or in the flammatory forci. Conclusion The various components in HCV can be expressed differently. The expressions of HCV-NS5 may be related to the mechanism of hepatitis C.