兔VX2肝癌模型制作方法的改进

Improvement of the rabbit model bearing vx2 transplanted hepatic carcinoma

目的建立合适的兔VX2肝癌动物模型,并与常规种植方法进行比较。方法30只新西兰实验兔随机分成三组,每组10只。三组实验动物在CT引导下,第1组采用瘤细胞悬液注射法,第2组采用瘤组织块悬液注射法,第3组(改进组)采用经皮瘤组织块注射法进行种植。种植后2周行CT扫描。结果三组种植成功率分别为30%、50%和90%,第1、3组间比较,差异有统计学意义。三组孤立肿瘤成功率为分别为10%、20%和80%,第1、3组间和第2、3组间分别比较,差异均有统计学意义。三组异位种植发生率分别为33%、20%和0。结论采用CT引导下经皮穿刺瘤组织块直接种植法成功率高,特别具有孤立肿瘤的发生率高、异位种植发生率低、动物损伤小等优点,是一项非常适用的免肝癌模型种植方法。

Objective To establish suitable metastatic rabbit vx2 hepatic carcinoma model, to compare the success rate of different tumor transplanted methods. Methods Thirty New Zealand white rabbits were randomly divided into three groups, 10 rabbits in each group. Group 1 ： Solution of the vx2 carcinoma cells were injected into left liver by syringe needle. Group 2 ： Solution of the tumor tissue masses was transplanted into liver. Group 3 （improvement group） ：vx2 carcinoma masses were injected into the liver using percutaneous puncture inoculation method. The operation was guided by CT scanner in each group. CT scanning was performed in all experimental animals 2 week after implantation. Results The success rates of transplanted carcinoma were 30% , 50% and 90% in group 1, group 2 and group 3 respectively, with the respective ectopic implantation rates of 33% ,20% and 0. The difference of success rates between group 1 and group 3 was statistically different. The success rates of single nodule implanted tumor were the highest in group 3（80% ） than in group 1 （10%） and group 2 （20%）. The difference between group 1 and group 2, group i and group 3 was statistically different. Conclusions It is successful to improve the rabbit model bearing vx2 metastatic hepatic carcinoma. And the method of percutaneous puncture carcinoma masses implantation guided by CT was a good method to set up the hepatic carcinoma models with a high success rates especially rates of single nodule implanted tumor, low ectopic implantation rates and little damage of animals.