高产纤维素酶的黑曲霉菌种的选育

Breeding of a High Cellulase Producing Strain Aspergillus niger

[目的]为纤维素的进一步开发利用奠定理论基础。[方法]选取菌落直径较大的黑霉菌株W1作为出发菌株进行紫外线诱变,检测诱变菌株在不同发酵时间的纤维素酶活力。[结果]黑曲霉W1经紫外线诱变10 min达到92%致死率。在试验检测的108 h范围内,随着培养时间在一定范围内延长,出发菌株W1菌株和从中筛选出的高产纤维素酶菌株NW1的酶活均有不同程度的提高,NW1在28℃100r/min培养84 h后酶活最高达到1.515 U/ml,W1培养84 h酶活达到0.958 U/ml,较出发菌株W1提高了1.58倍。之后2者的酶活都随着培养时间的延长稍有降低。利用0.1%刚果红染液初步检测诱变10 min的黑曲霉分泌纤维素酶情况,结果发现诱变菌株NW1分泌纤维素酶能力最强。[结论]黑曲霉诱变菌株具有较强的分泌纤维素酶的能力。

[ Objective] The aim was to lay the theoretical basis for the further development and utilization of cellulase. [ Method] Selecting Aspergillus n/ger WI with large diameter as original swaln,the cellulase activity in different fermentation time of the mutant strain was detected after ultraviolet mutation. [Result] The lethality of A. rdge W1 reached 92% through ultraviolet mutation for 10 min. During the 108 h detecting period,the enzyme activity of the original strain W1 and the screened high cellulase producing swain NWI increased with the prolonging of culture time in certain extent. The enzyme activity of NW1 was highest （1.515U/ml） after culture 84 h with 100 r/rain at 28 ℃ and that of W1 reached 0.958 U/ml after culture 84 h, and then that of the two strains decreased slightly with the prolonging of culture time in the following time. The secretion of cellulase from A. n/ge for 10 min mutation was detected initially by 0.1% Congo red dyeing solution and the result showed that the ability of ceUulase secreted by the mutant strain NW1 was strongest and increased 1.62 times than that of the original strain W1. [ Conclusion] The mutant strain of A. nige had strong ability of secreting cellulase.