花生中黄曲霉毒素检测方法的研究

Study on the Method for Determination of Aflatoxin in Peanut

[目的]建立了利用荧光光度法串联高效液相色谱法同时检测花生中4种黄曲霉毒素（B1、B2、G1和C2）的方法。[方法]样品经提取净化、荧光计初步筛选后，再利用高效液相色谱法进行确证分析。[结果]黄曲霉毒素B1、B2、G1、G2能达到完全的基线分离，检出限分别为0．3、1．0,0．3和1．0ug／kg，范围内线性关系良好，7〉0．999，回收率可达70％～96％，相对标准偏差低于9．4％。[结论]该方法快速、准确、灵敏且经济，能够满足花生中黄曲霉毒素B1、B2、G1、G2检验的需要。

[ Objective] Spectrophotofluorimetry with high performance liquid chromatography was applied to the deteimination of aflatoxin （ Bl, B2, G1 and G2 ） in peanut. [ Method] After extraction, the sample was purified by fluorometer. And high performanc liquid chromatography was used to analyze the sample. [Resuh] Baseline separation of aflatoxin B1,B2,G1 and G2 could be reached completely,with the limits of detection being 0.3, 1.0,0.3 and 1.0 ug/kg, respectively. The linear relationship within the range was good with γ 〉 0.999. The recovery rate could reach 70% - 96% .And the relative standard deviation was below 9.4%. [ Conclusion] The method was rapid,accurate,sensitive and economic.It could meet the need of the detection of aflatoxin B1, B2, G1 and G2 in peanut.