斑马鱼CDKAL1基因的生物信息学分析

Bioinformatics Analysis of CDKAL1 Gene from Danio rerio

[目的]深入了解斑马鱼CDKAL1基因的结构特点。[方法]运用生物信息学方法对斑马鱼CDKAL1基因的基因序列、编码蛋白的理化性质、结构及功能进行分析。[结果]分析结果显示,此基因一部分存在于细胞质,并存在一个跨膜结构域,没有信号肽。该基因编码蛋白质的变异不大;含有3个功能结构域,均为Erk D-domain,分别位于L189L、384、V315;在Y292位置有一磷酸化位点;有UPF0004超家族、Radical SAM超家族和MiaB结构域3个保守结构域;在脑、肌肉、肾脏中表达。推测该基因表达的蛋白质可能是酶、异构酶或者中间代谢产物,与胰岛素受体的作用有关。[结论]该研究为今后进一步研究人类Ⅱ型糖尿病提供了科学依据。

[Objective] The aim was to understand the structural characteristics of CDKAL1 gene from Danio rerio deeply. [Method] The sequence of CDKAL1 gene from D. rerio and the physical and chemical properties, structure and function of its encoding protein were analyzed using the bioinformaties method. [ Result ] The analysis result showed that the gene existed in the cytoplasm partially,had a trans-membrane domain and without signal peptide. The protein encoded by the gene had no significantly variation;it had 3 functional domains which was all Erk D-domain and located at L189,L384 and V315, resp. ; there was a phosphorylation site at the location of Y292; the protein had 3 conserved domains of UPF0004 superfamily, Radical SAM superfamily and MiaB domain;it expressed in brain, muscle and kidney. It was conjectured that the protein expressed by the gene could be enzyme, isomerase or intermediate metabolite and it had relation with the function of insulin receptor. [Conclusion] This research provided the scientific basis for the further study of human Type 2 diabetes for the future.