摘要
该试验采用PCR-RFLP技术对长白猪含凝血酶敏感蛋白模体的去解联金属蛋白酶(ADAMTS-1)基因部分序列进行克隆测序,在第7外显子发现1个C/G突变引起氨基酸改变。通过Pvu-ⅡRFLP基因分型,在长白猪群体中发现3种基因型(AA、BB、AB)。对不同基因型与部分繁殖性状进行关联分析,结果表明,ADAMTS-1基因与猪产仔数和产活仔数性状显著相关。
The nucleotide sequences in exon 7 of a disintegrin and metalloproteinase with thrombospondin motifs- 1 （ ADAMTS- 1 ） gene in l.andrace pig were obtained with cloning and sequencing by using PCR-RFLP technique in this experiment.Sequence alignment results showed that the C/G substitution in exon 7 induced amino acid change（Arg→ Pro）. AA,BB and AB genotypes were found in Landrace pig by Pru Ⅱ-RFLP. Correlatian analysis between gonotypes and reproductive traits indicated that ADAMTS-1 was significantly correlated with IS and NBA.
出处
《安徽农业科学》
CAS
北大核心
2008年第24期10374-10376,共3页
Journal of Anhui Agricultural Sciences
