RP-HPLC法测定湿生扁蕾中当药黄素和当药醇苷的含量

Determination of Swertisin and Swertianolin Contents in Gentianopsis paludosa(Munro)Ma by RP-HPLC

[目的]建立反相高效液相色谱法，同时测定湿生扁蕾中有效成分当药黄素、当药醇苷含量。[方法]采用Kromasil-C18（250mm×4．6mm，5um）色谱柱，流动相甲醇和水（含0．04％H3PO4），在0～20min甲醇：水由40：60～63：37线性梯度洗脱，流速0．8ml／min，检测波长254ml，柱温30℃。[结果]当药黄素、当药醇苷2种有效成分都在17min内均达到基线分离，当药黄素、当药醇苷的线性范围分别是0．338～2．025ug（r=0．9995）、0．275～1．650ug（r=0．9995），加标回收率为103．30％（RSD=3．20％）、99．60％（RSD=2．70％）。[结论]该方法快速，结果准确、可靠。

[Objective] The research aimed to establish a quantitative determination method of swertisin and swertianolin in Gentiopsis palulosa（Munro） Ma by RP-HPLC. [Method] The sample were separated on the column of Kromasil-C18 （4.6 mm× 250 nm,5 um） which eluted with methanol and water （0.04% phosphoric acid） .The ratio of methanol and water increased from 40：60 to 63：37 in 20 min with detected wave-length at 254 nm,flow rate at 0.8 ml/min, colunm temperature at 30℃. [Result] Two effective components were base-isolated. The linear ranges of swertisin and swertianolin were 0.338 - 2.025ug（ r = 0.999 5） ,0.275 - 1.650ug （ r = 0.999 5） respectively. Tile average recoveries were 103.30% （ RSD = 3.20% ） ,99.60% （ RSD .： 2.70% ）. [Conclusion] The method was rapid, precise and reliable.