退变椎间盘组织中TNF-α及IL-18的表达及意义

The expression and the meaning of TNF-α、IL-18 in the herniated intervertebral disc

目的:研究TNF-α、IL-18在突出与正常椎间盘组织中的表达,探讨该类细胞因子在椎间盘退变中的作用。方法:用Elisa法对34例椎间盘突出症患者椎间盘组织中TNF-α、IL-18进行检测,并取15例健康成人正常椎间盘用于阴性对照。结果:Elisa法证实突出椎间盘组织中TNF-α含量为82.18±21.91 pg/ml、IL-18含量为45.39±21.23 pg/ml;正常椎间盘组织中TNF-α含量为8.68±0.78 pg/ml、IL-18含量为3.58±2.84 pg/ml,两者差异有统计学意义(P<0.001)。结论:TNF-α及IL-18可能在椎间盘退变中发挥关键作用,并在临床对椎间盘突出的治疗和转归预后有重要意义。

Objective： To study the role of cytokines in the herniated and normal disc tissues,and then argue that this kinds of cytokines lead to the intervertebral cataplasia to some extent. Methods： Herniated intervertebral disc tissues were obtained from 34 patients undergoing surgery for persistent radiculopathy. The specimens were analysed Elisa treatment for the presence of TNF-α, IL- 18.As a control group 15 normal intervertebral disc specimens were harvested from 5 normal men and the same Elisa treatment detections were performed. Results：content of the TNF-α in the Herniated intervertebral disc is 82.18± 21.91 pg/ml,cantent of the IL-18 in the Herniated intervertebral disc is 45.39 ±21.23 pg/ml;content of the TNF-α in the normal intervertebral disc is 8.68±0.78 pg/ml; content of the IL- 18 in the normal intervertebral disc is 3.58 ± 2.84 pg/ml. Content of TNF-α and IL-18 has not increased apparently in control group, but in the experiment group increase rapidly（ P 〈0.01） was evidenced, significance. Conclusion： Herniated intervertebral disc cells can produce TNF-α,IL-18. These cytokines may be play a essential roll in the intervertebral disc degeneration and the pathophysiology of radiculopathy.