3-氨基丙基三乙氧基硅烷改性凹凸棒土固定化菠萝蛋白酶工艺研究

Study on the Immobilization Technology of Bromelin with Attapulgite Modified by 3-aminopropyltriethoxysilane

[目的]确定利用3-氨基丙基三乙氧基硅烷改性凹凸棒土（3-APTS-凹凸棒土）固定菠萝蛋白酶的最佳工艺条件。[方法]以3-APTS凹凸棒土为基质，在单因素试验的基础上，研究用固定菠萝蛋白酶的最佳工艺条件。[结果]确定菠萝蛋白酶固定化的条件为：0．4％戊二醛交联，温度38—44℃，pH值6．4，固定化时间4．5h，最终产品的酶活为3900U／g，酶活回收率达80％。[结论]利用3-APIS-凹凸棒土固定的菠萝蛋白酶稳定性和利用率显著提高。

[ Objective] The research aimed to optimize the optimum technology conditions of immobilizing bromelin with attapulgite modified by 3-aminopropyltriethoxysilane. [Method] Using 3-APTS-attapulgite as matrix, based on single factor test, the optimum technology conditions for immobilizing bromelin were studied. [Result] The immobilization conditions of bromelin were confirmed as follows： crosslinking with 0.4% glutaraldehyde at 38-44℃, pH 6.4, immobilization time of 4.5 h. The enzyme activity of the final products was 3 900 U/g and the enzyme recovery reached 80%. [ Conclusion] The stability and utilization rate of bromelin immobilized with 3-APTS-attapulgite were significantly improved.