鸡肉中磺胺嘧啶残留量的测定

Determination of Sulfadiazine Residue in Chicken

基于在酸性介质下磺胺嘧啶与碱性Luminol能产生化学发光，建立了化学发光微流动注射芯片测定鸡肉中磺胺嘧啶的方法。该芯片上微通道由激光雕刻机在聚甲基丙烯酸甲酯上雕刻而成，采用精确时间控制进样的方式检测，测定磺胺嘧啶线性范围为（4．0～50．0）×10^-8g／ml，检出限1×10^-8/ml，对2.0×10^-7g／ml磺胺嘧啶连续11次测定的相对标准偏差RSD为1．4％。

A micro-flow injection chemiluminescence system for detemination of sulfadiazine was described, based on luminol-sulfadiazine system. The flow channels were fabricated using laser on PMMA. The sampling time was controlled precisely by a timer. Under the selecting experimental conditions, the chemiluminescence intensity was linear with sulfadiazine concentrations of （4.0 -50.0） × 10^-8g/ml, the detection limit was 1.0×10^-8g/ml, and the relative standard deviation of 11 times of determination on 2.0×10^-7 g/ml sulfadiazine was 1.4%.