雪莲果叶片愈伤组织的诱导

Callus Induction of Smallanthus sonchifolius Leaves

[目的]研究雪莲果叶片愈伤组织诱导的最佳灭菌方法和最佳培养基。[方法]以雪莲果叶片为外植体,用浓度0.1%升汞浸泡不同时间,筛选最佳灭菌方法;以MS为基本培养基,比较不同浓度生长调节物质对愈伤组织诱导的影响。[结果]用浓度0.1%升汞溶液灭菌8 min,外植体污染率为54.55%,死亡率为3.64%;最佳诱导培养基为MS＋6-BA 1.0-1.5 mg/L＋NAA 0.5-1.0 mg/L。[结论]最佳灭菌方法为浓度0.1%升汞对叶片灭菌8 min;诱导愈伤组织的最佳培养基为MS＋6-BA 1.0-1.5 mg/L＋NAA 0.5-1.0 mg/L。

[ Objective] The experiraent was carried out to study the optimum sterilizing method and meditun in callus induction of Smallanthus sonchifolius leaves. [Method] The leaves were sterilized in 0.1% mercuric chloride at different times to seek out the optimum sterilizing method. MS medium with different concentrations of growth regulating substance was adopted to contrast their effects on callus induction. [ Result] Explants sterilized in 0.1% mercuric chloride for 8 rain had the best effect. The optimum medium for callus was MS ＋ 6-BA 1.0 - 1.5 mg/L ＋ NAA 0.5 - 1.0 mg/L. [ Conclusion] The optimum sterilizing method was sterilizing the leaves for 8min in 0.1% mercuric chloride. And the optimum medium for callus was MS ＋ 6-BA 1.0 - 1.5 mg/L＋ NAA 0.5 - 1.0 mg/L.