猪CSTB基因的克隆及多态性分析

Cloning and Analysis of Polymorphism on CSTB Gene in Pig

[目的]利用RT-PCR方法克隆猪CSTB基因exon2序列,对所得序列进行多态性分析。[方法]以大白猪cDNA为模板,采用RT-PCR克隆方法,首次获得猪exon2序列,提交GenBank收录(GenBank登录号:DQ534493)。[结果]通过RT-PCR方法,克隆测序得到的猪CSTB基因exon2序列与人、鼠的CSTB基因exon2序列同源性分别为80.39%与72.55%。对所得猪exon2序列进行多态性分析,发现第26和78个碱基位置发生突变,分别为A→T和C→T,其中第26个碱基为错意突变,导致氨基酸的改变。[结论]CSTB基因exon2序列在猪、人和鼠上相对保守。

[ Objective] CSTB gene exon2 sequence was cloned by RT-PCR. The sequence was carried on the polymorphism analysis. [ Method] CSTB gene exon2 sequence was cloned by RT-PCR in Yorkshire. Accession Number was DQ534493. [ Result ] By RT-PCR method, the pig exon2 had been cloned and sequenced. The sequence analysis showed that the pig CSTB gene and the human were homologous to 80.39% and the homology of rats of 72.55%. Two mutations were detected in the CSTB gene, such as the 26^th nucleotide of exon2 （A→T） and the 78th nucleotide of exon2 （C-→T）. The mutation （26^th nucleotide of exon2） changed the amino acids. [Conclusion]The CSTB gene.exon2, sequence was conservative in pig, human and rat.