不同处理方法对家兔超数排卵的影响

Effect of Different Treatment Methods on Superovulation of Rabbits

[目的]确定家兔稳定高效的超数排卵方法。[方法]PMSG试验组母兔颈部皮下注射PMSG 406、0、80 IU/只,FSH试验组母兔颈部下注射FSH 6次,每次间隔12 h,注射量递减,总用量分别为80、604、0 IU/只,注射PMSG、FSH后耳缘静脉注射HCG 100 IU/只,计算卵子回收率,比较2种处理方法的超数排卵效果。[结果]应用PMSG超排时,60 IU/只试验组的卵子回收率显著高于其他2组(P<0.05)。应用FSH递减法超排时,总用量为60 IU/只的试验组的卵子回收率显著高于其他2组(P<0.05)。FSH递减法试验组和PMSG试验组的卵子回收率无显著差异(P>0.05)。[结论]以60 IU/只的PMSG联合100 IU/只的HCG对家兔进行处理的超排效果最好,卵子回收率达70%。

[ Objective] The purpose was to confirm the stable and high efficient superovulation method for rabbits. [ Method] As for PMSG test group, the female rabbit was subcutaneous injected on its neck with PMSG 40, 60, 80 IU/pc, and for FSH test group, the female rabbit was FSH subcutaneous injected on its neck for 6 times at intervals of 12 h, the injection volume was in descending, and total dosage was 80, 60 and 40 IU per rabbit resp., after PMSG and FSH injection, the intravenous injection through the ear edge vein with HCG 100 IU per rabbit, the egg recovery rote was calculated, and the superovulation effect of two tieatment methods was compared. [ Result ] When using PMSG superovulation, the egg recovery rate for 60 IU/pe test group was significantly higher than the other 2 groups （ P 〈0.05）. As using FSH diminishing method for superovulation, the egg recovery rate for total dosage of 60 IU per rabbit in test group was obviously higher than the other 2 groups （ P 〈0.05）. The egg recovery rote for FSH diminishing method test group and PMSG test one had no evident difference （ P 〉 0.05）. [ Conclusion] The superevulation effect was the best with the treatment on rabbits in combination of 60 IU per rabbit in PMSG group with 100 IU per rabbit in HCG one and the egg recovery rate was up to 70%.