金银忍冬茎和叶中绿原酸的提取及含量测定

Extraction and Content Determination of Chlorogenic Acid in the Stems and Leaves of Lonicera maackii(Rupr.) Maxim

[目的]为寻找绿原酸新药源、综合利用金银忍冬提供科学依据。[方法]采用超声技术提取金银忍冬茎、叶中的绿原酸,高效液相色谱法对绿原酸含量进行测定。[结果]检测波长选择为327 nm。绿原酸进样量与峰面积在0.20-1.00μg范围内具有良好的线性关系。精密度试验的RSD为1.30%,说明仪器较精密。稳定性试验的RSD为1.15%。加样回收率试验的RSD为1.35%,平均回收率为98.52%。金银忍冬叶和茎中均含有绿原酸,经外标法测定其含量依次为：叶（1.54%）〉茎韧皮部（0.44%）〉茎木质部（0.42%）。[结论]超声提取法简单、快速,检测方法可行,测定误差较小,结果较准确,重现性好,可用于金银忍冬茎和叶中绿原酸的定量分析。

[Objective] The objective was to provide a scientific basis for the search of new resource of chloregenic acid and the multiple utilization for I.onicera maackii （Rupr.） Maxim. [ Method ] Chloregenic acid was extracted from the stems and leaves by ultrasonic technology and its content was determined by HPLC [ Result ] The detecting wavelength was selected as 327 nm, the sample volume of chlorogenic acid had good linear correlation with the peak area within 0.20 - 1.00μg. RSD value of precision experiment was 1.30%, and it indicated that the instrument was precise. RSD value of stability test was 1.15% and that of spotting recovery test was 1.35%, with the average recovery rate of 98.52%. The stems and leaves of L.M. Maxim all had ehlorogenic acid with the content from big to Small being leaves （1.54%） 〉 stem phloem （0.44%） 〉 stem xylem （0.42%）, which was determined by external standard method [ Conclusion] Ultrasonic technology was a simple, rapid and feasible method with small detemainate error, accurate result and good reproducibility, and could be applied to the quantitative analysis of chlomgenic acid in the stems and leaves of L.M. Maxim.