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GnRH激动剂Triptorelin对人卵巢癌细胞系SKOV3、OVCAR3生长抑制作用的研究 被引量：2

Study on the Inhibitory Effect of GnRH Agonist Triptorelin to Human Ovarian Cancer Cells
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摘要 目的观察GnRH受体在人卵巢癌细胞系SKOV3、OVCAR3中的表达,并研究GnRH激动剂(Triptorelin)对卵巢癌细胞生长的抑制作用。方法采用免疫组化SP法检测两种细胞系内GnRH受体的表达,分别应用不同浓度Triptorelin作用于细胞,MTT法检测细胞生长抑制率。结果人卵巢癌细胞系OVCAR3表达GnRHⅠ受体,SKOV3细胞系不表达GnRHⅠ受体,Trip-torelin可明显抑制GnRHⅠ受体阳性的OVCAR3细胞生长,对GnRHⅠ受体阴性的SKOV3细胞生长无明显抑制作用。结论GnRH激动剂Triptorelin通过与人卵巢癌细胞株GnRHⅠ受体结合,发挥抗增殖的作用。 Objective To observe the expression of gonadotropin releasing hormone receptor in SKOV3,OVCAR3 ovarian cancer cell lines,and investigate the inhibitory action of GnRH agonist. Methods Immunohistochemical SP was used to detect the GnRH receptor on SKOV3 and OVCAR3 cell lines.Different concentration of GnRH agonist were used to SKOV3 and OVCAR3 cell lines.The cells inhibition rates were detected by MTT technique. Results OVCAR cells show GnRH Ⅰ receptor immunoreactivity and triptorelin have growth inhibition action on these cells;but SKOV3 ceils didn＇t show GnRH Ⅰ receptor immunoreactivity,and triptorelin have no inhibiton action on these cells. Conclusion In ovarian cancer cell lines, the inhibitory effect of GnRH agonist was caused by it＇s combination to GnRH Ⅰ receptor.
作者 李敏 吴小华
机构地区 河北省人民医院妇科 白求恩国际和平医院妇产科
出处 《中国老年保健医学》 2008年第4期34-36,共3页 Chinese Journal of Geriatric Care
关键词 GNRH激动剂 SKOV3 OVCAR3 GNRH Ⅰ受体 GnRH agonist,SKOV3,OVCAR3,GnRH Ⅰ receptor
分类号 R737.31 [医药卫生—肿瘤]
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参考文献6

  • 1Castellón E,Clementi M.Hitschfeld C Effect of leuprolide and cetrorelix on cell growth,apoptosis,and GnRH receptor expression in primary cell cultures from human prostate carcinoma[].Can-cer Invest.2006
  • 2Chien CH,Lee CY,Chang TC,et al.Detection of gonadotropin-re-leasing hormone receptor and its mRNA in primary human ep-ithelial ovarian cancers[].International Journal of Gynecological Cancer.2004
  • 3Wang Y,,Matsuo H,Kurachi O, et al.Down-regulation of proliferation and upregulation of apoptosis by gonadotropin-releasing hormone agonist in cultured uterine leiomyoma cells[].European Journal of Endocrinology.2002
  • 4Choi JH,Choi KC,Auersperg N,et al.Differential regulation of two forms of gonadotropin-releasing hormone messenger ribonucleic acid by gonadotropins in human immortalized ovarian surface epitheliumand ovarian cancer cells[].Endocrine Related Cancer.2006
  • 5Grundker C,Gunthert AR,Millar RP,et al.Expression of gonadotropin-releasing hormoneⅡ (GnRHⅡ) receptor in human endometrial and ovarian cancer cells and effects of GnRH-Ⅱ on tumor cell proliferation[].The Journal of Clinical Endocrinology.2002
  • 6Grundker C,Schlotawa L,Viereck V,et al.Antiproliferative effects of the GnRHantagonist cetrorelix and of GnRH-II on human endometrial and ovarian cancer cells are not mediated through the GnRHtype I receptor[].European Journal of Endocrinology.2004

同被引文献30

  • 1张令宇,马文锋,杨栋.Hsp70、Fas和FasL在乳腺癌中的表达及其意义[J].中国肿瘤临床与康复,2005,12(3):193-196. 被引量：2
  • 2周士福,胡会华,李青国.Caspase-7、Caspase-3、Survivin与乳腺癌新辅助化疗的关系[J].山东医药,2007,47(27):12-13. 被引量：3
  • 3LONG Yu, TAO Min, LIU Shaojun, et al. Differential expres- sion of Gnrh2, Gthβ, and Gthr genes in sterile triploids and fer- tile tetraploids [ J]. Cell Tissue Res, 2009, 338 : 151-159.
  • 4JUNG H C, C BLAKE GILKS, NELLY AUERSPERG, et al. Immunolocalization of Gonadotropin Releasing Hormone (Gn- RH ) I, GnRH II, and Type I GnRH receptor during follicular de-velopment in the human ovary [ J]. J Clin Endocrinol Metabo, 2006, 91 ( 11 ) :4562-4570.
  • 5CHUDECKA G A, RZEPKA G I. Favorable effects of long-term therapy with gonadoliberin analogues in three patients with ad- vanced and recurrent ovarian cancer [ J]. Eur J Gynaecol On- col, 2009, 30 (5):535-538.
  • 6YANG X H, EDGERTON S, THOR A D, et al. Reconstitu- tion of caspase-3 sensitizes MCF-7 breast cancer cells to radia- tion therapy [J]. Int J Oncol, 2005, 26 (6) :1675-1680.
  • 7ALKASI O, MEINHOLD H I, ZAKI R. Long-term disease- free survival after hormonal therapy of a patient with recurrent low grade endometrial stromal sarcoma: a case report [ J]. Arch Gynecol Obstet, 2009, 279 ( 1 ) :57-60.
  • 8CASTELL1ON E, CLEMENTI M, H1TSCHFELD C. Effect of leuprelide and cetrorelix on cell growth, apoptosis, and GnRH receptor expression in primary cell cultures from human pros- tate carcinoma [ J ]. Cancer Invest, 2006, 24 ( 3 ) :261- 268.
  • 9WANG Y, MATSUO H, KURACHI O. Down-regulation of proliferation and up-regulation of apoptosis by gonadotropin-re- leasing hormone agonist in cultured uterine leiomyoma cells[J]. Eur J Endoerinol, 2002, 146 (3) :447- 561.
  • 10CHUDECKA G A, RZEPKA G I. Favorable effects of long-term therapy with gonadolibefin analogues in three patients with ad- vanced and recurrent ovarian cancer [ J]. Eur J Gynaecol On- col, 2009, 30 (5) :535-538.

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中国老年保健医学
2008年 第4期

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