山农一号在北海道黄杨组织培养中的应用

Application of Shannong No.1 in tissue culture of Euonymus japonicus Thunb

针对北海道黄杨(Euonymus japonicusThunb)组织培养过程中内生菌污染严重问题,使用山农一号进行杀菌和抑菌实验,结果表明:山农一号Ⅰ型能够有效对外殖体进行杀菌和抑菌,山农一号Ⅱ型添加在培养基中能有效抑制北海道黄杨组织培养过程中的内生菌.其中北海道黄杨茎段经山农一号Ⅰ型消毒后接种到含山农一号Ⅱ型的培养基中,杀菌、抑菌效果最佳,并且山农一号Ⅰ型和Ⅱ型对不定芽分化和外殖体的生长无抑制作用.

In order to prevent and control the severe pollution in tissue culture of tg.uonymus japonicus Thunb, Shannong No. 1 was applied to conducted the sterilization effect. The results showed that Shannong No. 1 type I could sterilize and eliminate endophytic bacteria effectivly, Shannong No. 1 type Ⅱ added in the medium to effectivly inhibit endophytic bacteria in the tissue culture of Euonymus japnicus Thunb. The stem segments of Euonymus japonicus Thunb. used Shannong No. 1 type Ⅰ disinfection after inoculation into contain Shannong No. 1 type Ⅱ in the culture medium, the effect of inhibitory and sterilization was optimum, and Shannong No. 1 type Ⅰ and Ⅱ there be no inhibitory to the bud differentiation and growth of explant.