邻菲啰啉-苯丙氨酸铜与DNA作用的研究

Interaction between DNA and Copper(Ⅱ) Complexes with Phenanthroline and Phenylalanine

用电化学法和电子吸收光谱法研究了配合物邻菲啰啉-苯丙氨酸铜与DNA的相互作用。电化学研究表明,配合物离子在电极上的反应过程主要由扩散过程控制,加入DNA使其峰电流降低,峰电位正移;同时,电子吸收光谱显示加入DNA使配合物离子吸收峰产生减色效应,表明此配合物能与DNA发生插入作用。通过电子吸收光谱,计算出铜配合物与DNA的结合常数为2.59×102L/mol,表明结合作用较弱。

The binding of DNA with the copper（ Ⅱ ） complex with phenanthroline and phenylalanine has been investigated by electrochemical techniques and absorption spectroscopy. The dectrochemical results show that the electrochemical reaction of the copper（ Ⅱ ） complex on the glass carbon electrode is controlled by the process of diffusion. In the presence of DNA, the values of cathodic peak potential shift positively and the values of cathodic peak current decrease compared with that in the absence of DNA. In the meantime, absorption spectral properties reveal that the absorption peaks exhibit hypochromicity when DNA was added. These experimental results indicate that the copper（ Ⅱ ） complex can intercalate weakly into the base-pairs of DNA and the binding constant is 2.59 × 10^2 L/tool calculated by the absorption spectra.