大剂量左氧氟沙星对兔肾功能影响的实验研究

Study on the effect of large doses levofloxacin to the kidney in rabbit

目的实验研究大剂量左氧氟沙星对兔肾功能的影响。方法将16只新西兰白兔随机分为四组:4只作为对照组,余按时间分为静脉滴注左氧氟沙星后1h组、2h组、3h组,每组4只。对照组给予生理盐水,其余3组均给于左氧氟沙星0.05g/kg,药物溶于50m l 0.9%N aC l溶液中。各组分别在给药后1、2和3h时应用彩色多普勒超声观察兔肾的大小、形态及内部回声的改变,应用彩色多普勒能量图及彩色血流观察兔肾血流灌注情况并测量兔肾动脉的血流参数。彩超观察完毕后于耳缘静脉在各个时点取血做生化检验,最后福尔马林灌注处死取材切片,使用光镜观察兔肾脏的形态学变化。结果左氧氟沙星组兔肾大小、内部回声、血流灌注及血流参数均无明显变化,各种生化指标均无明显变化,与对照组比较无统计学意义(P>0.05),光镜下左氧氟沙星组兔肾组织无明显异常。结论大剂量左氧氟沙星对兔肾功能无明显影响。

Objective To study the effect of large doses levofloxacin on the kideny. Methods Levofloxacin was injected intravenously at the dose of 0.05g/kg to 12 New Zealand White rabbit in the flowing groups ; the group divided in one-, two-, and three-hour. Ultrasonic examination was performed after one, two and three hours of injection. Then took the blood to make measurement. Finally took the tissue to make speci- men. Results Compared with the control group, there was no significant difference in the renal size, the BUN and Cr level of the kidney of all the treated groups （P〉0.05）. Light microscope showed the structure of the kidney was normal in the levofloxacin group. Conclusion The renal function was not effected significantly with the use of large doses levofloxacin.