CARB、aac(6′)-Ⅱ基因在铜绿假单胞菌烧伤患者分离株中流行

Drug Resistant Genes of CARB and aac(6′)-Ⅱ Prevailing in Pseudomonas aeruginosa Isolated from Burned Patients

目的了解铜绿假单胞菌烧伤患者分离株对β-内酰胺类、氨基糖苷类和磺胺药物耐药基因的分布情况。方法用聚合酶链反应(PCR)检测20株铜绿假单胞菌烧伤患者分离株β-内酰胺类(TEM、SHV、GES、CARB、I MP、VI M、DHA、oprD2)、氨基糖苷类(aac(6′)-Ⅰb、aac(6′)-Ⅱ、ant(3″)-Ⅰ、armA)和磺胺(sul1)等常见耐药基因。结果20株中,TEM阳性17株(85%),aac(6′)-Ⅰb阳性17株(85%),sul1阳性18株(90%),ant(3″)-Ⅰ阳性2株(10%),而CARB、aac(6′)-Ⅱ基因20株均为阳性(100%),oprD2基因均缺失(100%),其余基因为阴性,同一患者不同部位和时间分离株耐药基因可不同。结论CARB、aac(6′)-Ⅱ基因在铜绿假单胞菌烧伤患者分离株中流行,进行铜绿假单胞菌特别是其连续分离株的耐药基因检测非常必要。

OBJECTIVE To investigate drug resistant genes associated with beta-lactamases, aminoglycosides and sulfonamides in Pseudomonas aeruginosa isolated from burned patients. METHODS Common drug resistant genes associated with beta-lactamases, aminoglycosides and sulfonamides of 20 strains of P. aeruginosa isolated from burned patients were detected by PCR. RESULTS The positive rates of genes of TEM, aac（6＇）- Ⅰ b, sull and ant （3＂）-Ⅰ were 85%, 85%, 90% and 10%, respectively and genes of CARB and aac（6′）-Ⅱ were both positive （100%）. Genes of oprD2 were all absent and genes of SHV, GES, IMP, VIM, DHA and armA were all negative. Furthermore, drug resistant genes of strains isolated from different sites of one patient on different time could be different. CONCLUSIONS Drug resistant genes of CARB and aac（6′）-Ⅱ prevail in P. aeruginosa isolated from burned patients. Detections of drug resistant genes in P. aeruginosa isolated, especially continuously isolated from burned patients are essential.