复方邻苯二甲醛杀枯草芽胞杆菌黑色变种芽胞效果及机制研究

Germicidal Efficacy of Compound Ortho-phthalaldehyde Against Bacillus subtilis var. niger Spores and Its Mechanism

目的研究复方邻苯二甲醛杀枯草芽胞杆菌黑色变种芽胞的效果及其机制。方法采用悬液定量杀菌试验法研究复方邻苯二甲醛对枯草芽胞杆菌黑色变种芽胞杀灭效果,从蛋白质、DNA漏出、超微结构探讨其杀芽胞机制。结果在(20±1)℃条件下,含邻苯二甲醛(OPA)5000 mg/L的复方邻苯二甲醛作用枯草芽胞杆菌黑色变种芽胞30 min,平均杀灭对数值>5.00,枯草芽胞杆菌黑色变种芽胞经复方邻苯二甲醛作用后,有蛋白质漏出,但无DNA漏出;透射电镜观察发现复方邻苯二甲醛作用后,芽胞形状不规则,外壳电子密度深浅不均,甚至部分缺损,核心区偏位,出现电子透明区。结论复方邻苯二甲醛可有效杀灭枯草芽胞杆菌黑色变种芽胞,其机制是在苯扎溴铵的协同作用下,芽胞的渗透压改变,通透性增加,OPA更易进入细胞内导致蛋白质变性、漏出而起杀灭作用。

OBJECTIVE To study the germicidal efficacy and germicidal mechanism of compound ortho- phthalaldehyde （OPA） against Bacillus subtilis vat. niger spores. METHODS Suspension quantitative germicidal test was used to observe the germicidal efficacy of compound OPA against B. subtilis var. niger spores. The germicidal mechanism of compound OPA was studied in transudation of protein and DNA and ultra- microstructure. RESULTS The resuhs showed that at （20±1）℃, the logarithm mean reduction was greater than 5.00, after B. subtilis var. niger spores had been exposed to compound OPA for 30min. Some protein leaked from B. subtilis vat. niger spores treated with compound OPA for some time, hut there wasn＇t DNA. The uhrastructure showed that the shape was amorphic, the electronic density of the coat was unequal, the core leaned to one side or appeared the transmission area. CONCLUSIONS Compound OPA can effectively kill B. subtilis var. niger spores. Its mechanisms are that in accordance with benzolkonium bromide （Bromogeramine） the compound OPA can change the osmotic pressure of cell and increase cell permeability, then it can easily penetrate to their target sites within the cell.