体外培养的人子宫内膜细胞中GnRH及其受体的表达

Expression of gonadotropin-releasing hormone and its receptor in human endometrial cells cultured in vitro

目的探讨促性腺激素释放激素(GnRH)及其受体(GnRH-R)在体外培养的人子宫内膜异位症(EMS)和对照组在位子宫内膜细胞中的表达情况。方法用酶消化分离法培养EMS和对照组在位子宫内膜细胞;用免疫细胞化学法检测培养细胞GnRH和GnRH-R表达。结果17例标本15例培养成功,成功率88.2%;GnRH和GnRH-R在EMS和对照组在位子宫内膜培养细胞胞浆内均表达阳性,但经过3～4次传代后,GnRH不再表达,而GnRH-R持续表达。结论在位子宫内膜细胞的消化分离培养是一种简便易行的方法;GnRH-R在培养细胞中持续表达提示其可以用于研究GnRH类似物(GnRH-a)对子宫内膜的直接作用。

Objective To investigate the expression of gonadotropin-releasing hormone （GnRH） and its receptor （GnRH-R） in human endometriosis （EMS） and normal endometrial cells cultured in vitro. Methods Fresh eutopic endometrial specimens were collected during laparatoscopic operation from 10 EMS patients and 7 patients with other gynecological benign diseases aged 23-45, and digested by trypsin. The primary endometrial cells were cultured and passaged. Immunocytochemistry was used to detect the expression and localization of GnRH and GnRH-R. Resu its 15 of the 17 specimens （88.2%） were cultured successfully. Both GnRH and GnRH-R were expressed in all the endometrial cells, but the GnRH expression disappeared after passage of 2＋3 generations. Conclusion The continuous expression of GnRH-R proteins in endometrial ceils cultured in vitro indicates that the endometrial cells expressing GnRH can be used as a cell model to investigate the direct effect of GnRH-A on endometrium.