窗口期丙型肝炎病毒感染样品抗原检测意义分析

Analysis of Detecting HCVcAg during the Preseroconversion Window

目的对窗口期丙型肝炎病毒(HCV)感染样品检测核心总抗原(HCVcAg Trak-C)意义进行评价,考核国外丙型肝炎病毒核心抗原检测试剂盒的质量及可能的用途。方法用该试剂对8649份自然供血员及566份HCV可疑感染者血清进行核心总抗原检测,分析抗原的分布情况。与抗体试剂(Anti-HCV)、游离抗原(HCV free Ag)试剂、核酸(HCV RNA)试剂进行比较。并对检测数据进行统计分析。结果筛出157份核心总抗原和抗体同时阳性血清,抗体阴性而总抗原阳性血清5份。利用RNA试剂检测这5份血清HCV RNA阳性。利用HCV free Ag试剂检测这9215份血清,发现两份HCV游离核心抗原阳性血清,其中一份为抗原和抗体同时阳性血清,RIBA检测确证这份血清产生的是核心区抗体。另外一份为单独游离核心抗原阳性血清。结论由于检出了HCVcAg单独阳性的窗口期样品,认为利用HCV抗体试剂对血液进行筛查的同时,使用HCVcAg做必要的补充实验,可以减少因窗口期造成的漏检。

Objective To investigate the quality of an imported HCVcAg EIA kit and analyze this kit＇s different uses. Methods 9 215 sera were detected by HCVcAg EIA kit. The suspecious samples were confirmed with confirmed neutralization test and RIBA, and these results were analyzed in sucession. Results 158 positive HCVcAg sera were found by this kit. In the meantime, 5 was only positive HCVcAg during the preseroconversion window. Conclusion This kit can effectively detect hepatitis C core antigen during the preseroconversion window. It is suggested that the manufacture should establish the system of correct CUTOFF.