不同干旱程度及复水对水稻B优827开花期的生理影响

Effects of Water Stress and Rewatering on Physiological Characters of Rice B You 827 at Flowering Stage

于开花期对籼型杂交水稻B优827和中度抗旱品种冈优725(对照)进行3个水平的水分胁迫处理,之后复水。研究光合速率、活性氧(ROS)、渗透调节物质、谷胱甘肽(GSH),抗坏血酸(AsA)和抗氧化酶活性等生理指标及其产量性状的变化。结果表明,B优827在轻度水分胁迫、重度水分胁迫下都表现为抗干旱能力比冈优725高;轻度水分胁迫下,随着胁迫时间的增加,光合速率、GSH和AsA含量下降,活性氧和渗透调节物质含量及抗氧化酶活性上升。而重度水分胁迫下,随着胁迫时间的增加,光合速率、GSH和AsA含量下降迅速,活性氧和渗透调节物质含量及抗氧化酶活性比轻度胁迫下增加的快,机体受到严重的损伤。不同程度水分胁迫处理收获后其产量大小分别为正常水分>轻度胁迫>重度胁迫。复水后测定结果显示,机体代谢会逐渐恢复正常。

Three water stress treatments were set to investigate the effects of water stress on Pn, reactive oxygen species （ROS）, osmosis, glutathione （GSH）, ascorbic acid content（AsA）, antioxidative enzyme activities and the yield characters of two rice hybrids （B You 827 and Gongyou 725） at flowering stage. The result showed that the drought resistance of B You 827 was better than that of Gangyou 725 in both light and severe water stresses. In light water stress, followed by continue of water stress, Pn, the content of GSH and AsA decreased, the content of ROS and osmosis, antioxidative enzyme activities increased in rice leaves. In severe water stress, however, followed by continue of water stress, Pn, the content of GSH and AsA decreased quickly, the content of ROS and osmosis, antioxidative enzyme activities in rice leaves were higher than those in light water stress, leading to the severe damage of organs. After harvest, the magnitude of yield treated with different water stress is summarized as： CK〉light〉severe. After rewatering, the body metabolism could recovere to normal level.