鸡新城疫/传染性支气管炎二联油乳剂 灭活疫苗试验研究报告(Ⅱ)——疫苗制备及其免疫效果试验

Studies on Inactivated ND IB Oil-adjuvant Bivalent Vaccine(Ⅱ)- Vaccine Preparation and its Immunity Effects

根据ＮＤＶ、ＩＢＶ二各病毒混合毒种的最适稀释比例，将混合毒种接种９－１０龄鸡胚尿囊腔内（简称同胚培养），使接种鸡胚９６ｈ二种病毒毒价同时达到高峰，０．０１ｍｌ尿囊液含：ＮＤＶ≥１０＾８ＥＩＤ５０、ＩＢＶ≥１０＾７ＥＩＤ５０。收获同胚培养的胚毒，制备同胚培养的胚毒，制备油乳剂灭活疫苗。用三批疫苗分别免疫１８日龄雏鸡，免疫后４周二联疫苗的有效抗体分别为：ＮＤ血凝抑制抗体（ＨＩ）≥１：１６。

NDV and IBV were mixed according to the optimal dilution proportion, then the viruses were inoculated into chicken embryos at 9 or 10-dayage. the titres of two viruses both reached the peak at 96h, the allantoic fluid contained NDV≥108EID50 and IBV ≥107EID50 per 0. 1 ml. The viruses were harvested, and the inactivated oil-adjuvant vaccines were prepared. The chickens were vaccinated at 18-day-age,ail chickens had HI titres of ND ≥1:16 and neutralizing antibody of IB≥l:8 in 4 weeks after vaccination. The duration of immunity lasted 16 weekd, and the protection rate was 8/8(ND) and 6/8(IB) respectively.