消毒剂灭活猫杯状病毒中和剂鉴定试验方法

NEUTRALIZATION METHOD OF DISINFECTANT IN INACTIVATION OF FELINE CALICIVIRUS

目的研究猫杯状病毒作为指标,评价化学消毒剂灭活病毒的中和剂试验方法。方法采用细胞感染法培养病毒,以噬斑计数法测定含氯消毒剂对猫杯状病毒的杀灭效果。结果采用本研究设计的中和剂试验方法所选出的中和剂能完全中和有效氯含量为1000 mg/L的该消毒剂,但其中和产物对培养细胞有毒。用EBSS溶液作2.5倍稀释后,可消除中和产物对试验细胞的毒性,使其可以用于病毒灭活试验的中和剂。以含有效氯1000 mg/L的次氯酸钠消毒液对猫杯状病毒作用1 min,平均灭活率为99.16%;作用5 min,平均灭活率为99.99%。结论以细胞感染法的噬斑计数测定消毒剂灭活猫杯状病毒的中和剂试验方法,达到操作简单、效果可靠的目的,可作为消毒试验参考。

Objective To evaluate the method of neutralization of chemical disinfectant for inactivation of virus with feline calicivirus （FCV） as the indicator. Methods The method of infecting cells was adopted to cultivate the virus. The plaque forming unit count was used to measure the efficacy of chlorine - containing disinfectant in inactivation of feline calicivirus. Results The neutralizer used in this test could neutralize the disinfectant containing available chlorine 1000 mg/L, but the neutralization product was toxic to the CrFK cells for culturing. After being diluted to 2.5 times by EBSS, the toxicity of the neutralization product to the CrFK cells could be eliminated. The average killing rate of FCV exposed to sodium hypochlorite containing 1000 mg/L available chlorine for 1 min was 99.16%, and the average killing rate was 99.99% after exposure for 5 min. Conclusion The method of virus infecting cells and plaque forming unit count method are simple and reliable for determining neutralization of disinfectant in virucidal test for FCV. The methods can be used as the reference for the disinfection experiment.