几种医院感染病原菌抗药基因检测及其对聚维酮碘抗性研究

DETECTION OF DRUG-RESISTANT GENE OF A FEW NOSOCOMIAL INFECTION PATHOGENIC BACTERIA AND STUDY ON ITS RESISTANCE TO POVIDONE IODINE

目的研究几种临床分离病原菌抗药基因携带情况及其对聚维酮碘的抗力水平。方法采用PCR方法和肉汤稀释法，检测几种病原菌qacEΔ1-sulⅠ基因和最小抑菌浓度。结果检测临床分离的4种30株病原菌，有13株qacEΔ1-sulⅠ基因阳性，携带率为43．33％。聚维酮碘对7株铜绿假单胞菌的MIC值为500～1000mg／L，标准菌株为1000mg／L；对7株大肠杆菌和7株鲍曼不动杆菌的MIC值均与标准菌株相同，均为1000mg／L；对9株金黄色葡萄球菌的MIC值有5株为1000mg／L，高于标准菌株的500mg／L。结论临床分离的30株病原菌抗药基因携带率为43．33％，携带抗药基因的菌株与其对聚维酮碘的抗力关系不明显。

Objective To study the drug - resistance gene carried by a few clinically isolated pathogenic bacteria and its level of resistance to povidone iodine. Methods PCR method and broth dilution method were used to examine qacEΔ1-sulⅠ gene of several pathogenic bacteria and minimal inhibitory concentration （MIC）. Results Of the clinically isolated 30strains of pathogenic bacteria, 13 were found to be qacEΔ1-sulⅠ gene positive, with drug - resistance gene carrying rate of 43.33%. The MIC value of povidone iodine for 7 strains of Pseudomonas aeruginosa was 500-1000 mg/L and for standard strain was 1000 mg/L. The MIC values of povidone iodine for both 7 strains of Escherichia coli and 7 strains of Acinetobacter baumannii were 1000 mg/L, which was same as that for the standard strain. The MIC value for 5 of 9 strains ofStaphyloccocus aureus was 1000 mg/L, which was higher than 500 mg/L for the standard strain. Conclusions Drug - resistance gene carrying rate of the clinically isolated 30 strains of pathogenic bacteria was 43.33%. There is no significant correlation between carrying the drug - resistance gene and the resistance to povidone iodine.