海洋石油烃降解细菌T_1和T_2的分子鉴定分类

Molecular identification and classification of marine hydrocarbon degrading bacteria T_1 and T_2

从天津滨海潮间带被石油烃严重污染的沉积物(干样含油量0.2 g/g)中,筛选分离出能够以柴油为唯一碳源生长的细菌,对其中生物量大、单株菌降解效率较高的两株细菌 T_1和 T_2进行16S rDNA 克隆,通过测定和比较16S rDNA 的部分序列对这两株细菌进行分子鉴定,以期用于石油污染的微生物修复中。结果表明,T_1与深红红螺菌(Rhodospirillum rubrum)的同源性为89%,T_2与施氏甲单孢杆菌(Pseudomonas stutzeri)的同源性为99%。

Hydrocarbon degrading bacteria were obtained from oil-polluted sediments in the intertidal zone of Tianjin seashore by a soil enrichment procedure using diesel fuel as carbon source. The 16S rDNA amplified from 2 strains of these isolations, T1 and T2, which have a high biomass and a hydrocarbon degrading capability, was cloned. According to molecular criteria, nucleotides sequence of 16S rDNA was determined and analyzed. The results indicate that strain T1 is similar to Rhodospirillum rubrum （89%） and strain T2 is similar to Pseudomonas stutzeri （99%）.