网箱养殖石斑鱼病毒性神经坏死病流行调查

Epidemiologic investigation of viral nervous necrosis in cage-cultured rockfishes in Xiamen

厦门海水网箱养殖石斑鱼春、夏季节暴发性疾病经过流行病学调查和病原检测,确认主要病原为病毒性神经坏死病毒,病鱼外观表现典型的神经异常症状。应用逆转录聚合酶链式反应(RT-PCR)技术检测5种石斑鱼:赤点石斑鱼(Epinephelus akaara)、青石斑鱼(E.awoara)、云纹石斑鱼(E.moara)、紫石斑鱼(E.lanceolatus)、马拉巴石斑鱼(E.malabaricus)。检测76条石斑鱼,检出率90%;病鱼的脑、视网膜、肾脏、肝脏、脾脏等组织均能检出神经坏死病毒(NNV),以脑和视网膜检出率最高,肾脏次之,肝脏、脾脏检出率较少,鳃未检出。

A serious epidemic disease usually breaks out in spring and summer in cage-cultured rockfishes in Xiamen, Fujian province, China, and infected fshes appear abnormal in the nervous system. After epidemiologic investigation and pathogenic assay, the nervous necrosis virus （NNV） was identifed to be the main pathogen. Seventy-six rockfishes, beloging to five kinds： Epinephelus.akaara, E. awoara, E. moara, E. lanceolatus and E. malabaricus, were examined using reverse transcription polymerase chain reaction （RT-PCR） assay, and NNV was detected in 90% of the rockfishes examined. In infected rockfishes, NNV could be detected in brain and retina most frequently, followed by kidney, less in liver and spleen, but could not be detected in branchia.