摘要
目的建立知柏地黄丸浓缩丸中丹皮酚的含量测定方法。方法采用高效液相色谱法,KromasilKR100-5C18(250×4.6mm)色谱柱,流动相:甲醇-水(70:30),流速:1.0mL·min^-1,检测波长:274nm。结果丹皮酚在8.168~163.36μg·mL^-1。范围内线性关系良好。(r=0.9999,n=6),平均回收率99.12%,RSD=0.52%(n=6)。结论本法快速,简便,准确,重复性好,可作为该丸剂的含量测定方法。
OBJECTIVE To establish a method for determination Of paeonol in Zhibaidihuang concentrated pill. METHODS Using a HPLC method with kromasil KR100-5C18(250 × 4.6mm)column, the mobile phase was methanol-water (70 : 30) at a flow rate of 1.0mL·min^-1, the UV detection wavelength was 274nm. RESULTS In the scope of 8. 168-- 163.36μg·mL^- 1, the contents of paeonol had good linear relationship with their peak area (r = 0.9999, n = 6).The average recoveries were 99.12%, RSD = 0.52% (n = 6) . CONCLUSION The results showed that the method was rapid, simple, excellent precision, and could be used for the method of determination.
出处
《海峡药学》
2008年第8期31-33,共3页
Strait Pharmaceutical Journal