HPLC法测定知柏地黄丸浓缩丸中丹皮酚的含量

Determining the contents of paeonol in Zhibaidihuang concentrated pill by HPLC

目的建立知柏地黄丸浓缩丸中丹皮酚的含量测定方法。方法采用高效液相色谱法，KromasilKR100-5C18（250×4．6mm）色谱柱，流动相：甲醇-水（70：30），流速：1．0mL·min^-1，检测波长：274nm。结果丹皮酚在8．168～163．36μg·mL^-1。范围内线性关系良好。（r=0．9999，n=6），平均回收率99．12％，RSD=0．52％（n=6）。结论本法快速，简便，准确，重复性好，可作为该丸剂的含量测定方法。

OBJECTIVE To establish a method for determination Of paeonol in Zhibaidihuang concentrated pill. METHODS Using a HPLC method with kromasil KR100-5C18（250 × 4.6mm）column, the mobile phase was methanol-water （70 ： 30） at a flow rate of 1.0mL·min^-1, the UV detection wavelength was 274nm. RESULTS In the scope of 8. 168-- 163.36μg·mL^- 1, the contents of paeonol had good linear relationship with their peak area （r = 0.9999, n = 6）.The average recoveries were 99.12%, RSD = 0.52% （n = 6） . CONCLUSION The results showed that the method was rapid, simple, excellent precision, and could be used for the method of determination.