摘要
目的建立反相高效色谱法测定盐酸尼非卡兰粉针的含量。方法采用HypersilODS柱（4．6mm×250mm，5μm）．检测波长269nm。流动相为甲醇-0．02mol·L^-1醋酸铵（60：40）；流速：1．0mL·min^-1。结果盐酸尼非卡兰在9．35--95．7μg·mL^-1浓度范围内线性关系良好（r=0．99998）。平均回收率为101．29％，RSD=0．28％。结论本法快速，简便．准确，重复性好。
OBJECTIVE To develop a method for determination of nifekalant hydrochloride in injects. METHODS The chromatographic column was Hypersil ODS. The mobile phase was methanol-0.02mol·L^-1 ammonium acetate （60：40）.The flow rate was 1.0 mL·min^-1 and the detection wavelength was 269nm. RESULTS In the scope of 9.35--95.7μg·mL^- 1, the contents of nifekalant had good linear relationship with their peak area（r = 0. 99998）. The average recoveries were 101.29 %, RSD = 0.28 % . CONCLUSION The results showed that the method was rapid, simple, excellent precision.
出处
《海峡药学》
2008年第8期33-34,共2页
Strait Pharmaceutical Journal
