摘要
目的HPLC中药成分检测实验的系统优化设计。方法结合Taguchi方法和ANOVA方法,兼顾色谱信号的最小变异和最优分离品质,寻找出影响HPLC实验过程较显著的因子,以及色谱条件的最佳化趋势。结果以感冒止咳糖浆中黄芩苷的检测为例,系统设计实验,确定流动相甲醇百分比为影响检测过程最显著的因子,并优选出最佳色谱条件趋势为柱温30℃,流速0.6mL.min-1,流动相甲醇百分比为45%;结论本法科学地设计实验和快速有效提升实验效率与品质,可作为优化HPLC中药成分检测条件的有效方法。
To optimize the HPLC chromatogram conditions for Chinese medicine ingredients analysis, this paper presents the technique of HPLC experiment design optimization based on Taguchi and ANOVA methods. The detection of baiealin in Ganmao Zhike Syrup is used as an example for this study, and the generated optimal chromatogram conditions are 30℃ column temperature, 0.6mL·min^-1 flow rate, and 45 % mobile phase methanol percentage. It is shown that the proposed technique can be used to improve the efficiency and performance of the experiments, and is feasible to optimize the HPLC Chinese medicine ingredients detection conditions.
出处
《海峡药学》
2008年第8期45-47,共3页
Strait Pharmaceutical Journal
