转基因棉花对根际土壤微生物多样性的影响

Effect of Transgenic Cotton on Microbial Community Diversity in Rhizosphere Soil

本研究应用PCR、T/A克隆、RFLP等分子生物学研究方法分析了两种大田栽培的转基因抗虫棉(SGK321、中棉所41)苗期、花铃期、衰老期根际土壤微生物特异性DNA序列变化,探讨转基因棉花种植对根际土壤微生物区系组成多样性的影响。16SrDNA和5.8SITSPCR扩增产物经T/A克隆分别得到1066、563个阳性克隆,根据酶切图谱进行聚类分析。结果表明,相同生育期的棉花根际土壤微生物区系相似性远高于转基因棉花根际微生物间的相似性。这表明转基因棉花对根际土壤微生物的影响不明显,棉花根际土壤微生物区系差异主要受生育期影响。

In order to evaluate effect of transgenic cotton on microbial community diversity in rhizosphere soil, 16S rDNA and 5.8S ITS genebased molecular analysis of DNA directly extracted from rhizosphere soil of two transgenic cotton cuhivars （SGK321 and ZHMIAN41） growing in field was studied by PCR, T/A clone and RFLP techniques at different growth period, compared to the non-transgenic parental cuhivars （SHYUAN321 and ZHMIAN23）. The PCR products of 16S rDNA and 5.8S ITS amplified with universal primers of bacteria and fungi were cloned by T/A clone techniques, and 16S rDNA and 5.8S ITS clones were 1 066 and 563, respectively. Both 16S rDNA and 5.8S ITS of each clone were digested with two restriction endoenzymes. Analysis of fingerprinting using the UPGMA clustering algorithm showed that the similarity of the microbial community composition of cotton at same growth period was much higher than that of transgenic cotton. It may be con- cluded there was little difference in terms of impacts between the two transgenic cotton cultivars and their non-transgenic parental cuhivars, while the main impacts on microbial community composition in rhizosphere soils were likely due to different growth period.