夏枯草提取物对人淋巴瘤Raji细胞增殖的影响及机制探讨

Effect and mechanism of Prunella vulgaris L extract on proliferation of human Burkitt lymphoma cell line Raji

目的观察夏枯草提取物对人Burkitt淋巴瘤Raji细胞增殖的影响,并探讨其机制。方法Raji细胞分别加入不同浓度夏枯草提取物进行培养,MTT法检测Raji细胞增殖抑制率,倒置显微镜观察细胞学形态,琼脂糖凝胶电泳观察Raji细胞DNA凋亡情况;流式细胞仪检测Raji细胞凋亡率。结果不同浓度的夏枯草提取物对Raji细胞增殖有明显的抑制作用,并呈剂量依赖性,半数抑制浓度(IC50)为(18.01±0.92)μg/ml;随夏枯草提取物作用时间的延长Raji细胞DNA凋亡条带增宽变亮,细胞凋亡率升高。结论夏枯草提取物可抑制Raji细胞增殖,其机制可能与诱导细胞凋亡有关。

Objective To observe the effects of the extract from Prunella vulgaris L on human Burkitt lymphoma cell line Raji. Methods The effects of the extract from Prunella vulgaris L on Raji cells proliferation was detected with MTT. Apoptosis of Raji cells was observed by cell morphology and agarose gel electrophoresis. The apoptosis rate of Raji cells were detected by flow cytometry. Results The different doses of the extract from Prunella vulgaris L could remarkably inhibit the proliferation of Raji cells with dose dependent, and the IC50 was （18.01 ± 0.92） μg/ml. The apoptosis strap was widen and brighten, and the apoptosis rate heightened with time. Conclusion The extract from Prunella vulgaris L can inhibit the proliferation of Raji cells by inducing apeptosis probably.