光动力法诱导大鼠视网膜新生血管动物模型的建立

Laser-induced model of retinal neovaxcularization in rats

目的:建立视网膜静脉阻塞诱导新生血管生成的动物模型,为视网膜新生血管性疾病的研究提供条件。方法:健康成年SD大鼠50只,常规麻醉后,尾静脉注射虎红,532nm激光光凝视网膜主要静脉,建立视网膜新生血管动物模型。光凝术后2wk,采用心内注射FITC-dextran,视网膜铺片法观察新生血管生成的情况。结果:光凝手术时,可见视网膜静脉内明显黑色血栓形成。光凝后第2d发现视网膜主要静脉迂曲粗大、可见栓塞引起出血。2wk后FITC-dextran心内灌注视网膜铺片显示光凝眼视网膜血管形态和分布发生明显改变,失去正常的放射状和网状结构,出现大量新生血管芽和新生血管枝,视网膜血管杂乱、荧光素渗漏。结论:成功的构建了光动力诱导视网膜静脉阻塞的动物模型,并使用FITC-dextran心内灌注视网膜铺片对视网膜血管进行造影,从整体显示视网膜血管和新生血管的形态。该模型稳定性和重复性好,是研究视网膜新生血管的较为理想的动物模型。

AIM： To establish construction laser-induced retinopathy animal model and to provide the basis for further studying on retinal neovascularization. METHODS： Anesthetized fifty SD rats were injected of Rose Bengal by tail vein injection. 532nm laser photocoagulation was given to the retinal vein. Two weeks after transfection, the neovessels were observed by FITC- dextran retinal angiography. RESULTS： During the photocoagulation, we found black thrombogenesis in the retinal vein, distant vein expand. The day after photocoagulation, we examined the rat fundus and found that retinal vein expand greatly. Two weeks after transfection, large part of neovascularization and FITC leakage in the group of coagulation could be observed by FITC-dextran retinal angiography. Those in the group of blank intervention retinal vessel were normal. CONCLUSION ： Laser-induced retinopathy animal model is an useful model in the study of the neovascular retinopathy.