细胞角蛋白19片段、癌胚抗原、糖链抗原125联合检测在肺癌诊断中的应用

Combined Determination of CYFRA21-1, CEA and CA125 in Lung Cancers

目的:研究与肺癌相关的3种肿瘤标志物细胞角蛋白19片段(CYFRA21-1)、癌胚抗原(CEA)、糖链抗原125(CA125)在肺癌诊断方面的临床应用价值。方法:采用化学发光法分别检测70例肺癌患者、46例肺部良性疾病患者和37例健康对照者血清中的CEA、CA125水平,用ELISA方法检测上述人群血清CYFRA21-1水平。用统计软件SPSS10.0评价此3种肿瘤标志物在肺癌辅助诊断方面的临床应用价值。结果:肺癌患者血清CYFRA21-1、CEA、CA125水平高于肺良性疾患者和健康体检人群(P<0.01);腺癌患者血清CEA、CYFRA21-1水平高于鳞癌(P<0.05);非小细胞肺癌患者血清CEA水平高于小细胞肺癌患者(P<0.05)。CYFRA21-1对肺癌辅助诊断的特异性最好;CYFRA21-1+CEA+CA125联合检测的敏感性最高。结论:3种肿瘤标志物在肺癌诊断中具有一定的临床应用价值,联合检测可显著提高肺癌诊断的敏感性和有效性。

Objective： To investigate the clinical value of combined determination for three biomarkers, cytokeratin-19-fragments （CYFRA21-1）, earcinoembryonic antigen （CEA） and cancer antigen 125 （CA125）, in patients with lung caneer. Methods： Chemiluminescence immunoassay was used to measure the serum levels of CEA and CA125 and ELISA was used to measure CYFRA21- 1 in 70 patients with lung cancer, 46 with benign pulmonary diseases and 37 heahbv adults. All data were analyzed by SPSS10.0. Results： The serum levels of CYFRA21-1, CEA and CA 125 were significantly higher in patients with lung cancer than in those with benign pulmonary diseases and in normal control （P〈0.O1）. Higher levels of CEA and CYFRA21-1 were more associated with adenocarcinoma than with squamous carcinoma, as did higher level of CEA with non-small cell carcinoma than with small cell lung cancer （both P〈 0.05）. For diagnosis of lung cancer, highest specificity was found with CYFRA21-1 alone, while the highest sensitivity was achieved with combined determination of CYFRA21 - 1, CEA and CA125. Conclusion： While the three tumor markers may offer clinical benefits for diagnosis of lung cancer, combined determination would result in significantly improved sensitivity and efficency.