盆底器官脱垂患者主韧带和阴道壁Ⅲ型胶原代谢变化

Profiles of Collagen Type Ⅲ in Cardinal Ligament and Vaginal Walls of Patients with Pelvic Organ Prolapse

目的:探讨盆底器官脱垂(POP)患者Ⅲ型胶原代谢与POP的发病关系。方法:收集本院POP患者和因子宫良性病变行手术治疗的非POP患者各13例,术中取主韧带及阴道前壁组织,免疫组化方法测定Ⅲ型胶原含量,ELISA法测定Ⅲ型胶原前胶原氨基端肽PⅢNP的含量。结果:POP组主韧带及阴道前壁组织的Ⅲ型胶原含量低于对照组(P<0.05),PⅢNP含量高于对照组(P<0.05)。结论:POP患者韧带和盆底筋膜组织Ⅲ型胶原含量减少可能是由于胶原降解的增加,影响盆底支持组织的强度和弹性,导致POP发生。

Objective： To determine the metabolic profiles of type Ⅲ collagen leading to development of pelvic organ prolapse （POP） in female patients. Methods： Each 13 women who received surgical treatment in this hospital for POP （POP group） and benign uterine disorders （control group） were studied. Bioptic samples of the cardinal ligament and anterior vaginal wall were obtained during operations, subsequently assessed for contents of type Ⅲ collagen using immunohistoehemieal techniques and that of N-terminal procollagen Ⅲ propeptide （P Ⅲ NP） as a marker of type Ⅲ collagen metabolism using ELISA. Results： The POP group had significantly lower amount of type Ⅲ collagen and higher level of P Ⅲ NP as compared with the controls （both P〈0.05）. Conclusion： The reduced type Ⅲ collagen and higher level of PⅢ NP in POP women suggested an increased collagen breakdown, which may negatively affect tissue strength and elasticity of the pelvic floor and may lead to POP.