大鼠耳蜗发育过程中生存素和半胱氨酸天冬氨酸蛋白酶3的表达

Expression of survivin and caspase-3 during the development process in rat cochlea

目的探讨大鼠耳蜗形态学发育过程中凋亡相关因素的作用。方法通过免疫组化蛋白定位以及逆转录一聚合酶链反应（RT-PCR）技术检测生存素（survivin）和半胱氨酸天冬氨酸蛋白酶3（caspase-3）在胚胎期及出生后Wistar大鼠耳蜗中的表达情况。结果survivin和caspase-3在细胞增殖期广泛表达于即将分化发育形成Corti器的蜗管底壁，在细胞分化期表达逐渐增强；survivin在细胞分化末期达到高峰，持续到细胞发育成熟期开始之后下降，在成熟的corti器毛细胞中仍有较强表达；而caspase-3在细胞发育成熟期开始时达到高峰，以后逐渐下降，直至表达消失。结论survivin和caspase-3在大鼠耳蜗发育过程中表达范围相似，但表达量在时间上并不同步，survivin的表达高峰早于caspase-3，二者相互作用共同调控细胞凋亡，促进耳蜗形态结构的成熟。

Objective To explore the role of some apoptosis regulators during the development in rat cochlea. Methods The morphological development process of cochlea was observed in Wistar rat aged between embryo day 13 to postnatal day 14 in this experiment. Survivin and caspase-3 were respectively detested at protein and mRNA levels by immunohistochemistry and reverse transcription polymerase chain reaction（RT-PCR）. Results The expression of survivin and caspase-3 located in the bottom wall of the cochlear duct. Not only they widespread in the cell proliferation, but also they gradually enhanced in the cell differentiation. Both of them had a crest-time, and survivin was prior to caspase-3. In organ of corti during adult time, caspase-3 was not present and survivin was only expressed. Conclusions During the development of the rat cochlea, both of them had similar location and trend. But they were derangement. This showed that both of them participated in the cochlear morphological development. It was suggested that the interaction between survivin and caspas-3 regulated the apoptosis, promoted the cochlear morphological development.