骨髓间充质干细胞移植重建极重度放射损伤小鼠造血功能

Effect of bone marrow mesenchymal stem cells transplantation in reconstructing hematopoiesis function of mice with critical irradiation injury

目的:探讨小鼠骨髓间充质干细胞(mouse bone marrow mesenchymal stem cell,mMSC)单独输注对重度放射损伤小鼠造血功能重建的影响。方法:将体外培养扩增的雄性C57BL/6小鼠骨髓间充质干细胞输注给受致死剂量全身照射(8 Gy)的雌性C57BL/6小鼠,检测移植后外周血象、骨髓有核细胞数、病理变化、粒-单核细胞系祖细胞集落(CFU-GM)计数及性染色体比例,以未输注MSCs的小鼠作对照。结果:对照组小鼠(n=6)在照射后20d内全部死于造血功能衰竭;MSCs组移植后血象明显下降,但2周后迅速恢复,28d时恢复到照射前的60 %左右,42d外周血象基本恢复。MSC促进骨髓有核细胞数及CFU-GM快速恢复,有利于骨髓组织学的明显恢复改善。移植后42 d ,仍可在受致死量照射的受体鼠内检测到供体MSC,但不能长期植入。结论:小鼠骨髓间充质干细胞单独输注可促进重度放射损伤小鼠的造血功能恢复,其植入时间长短可能与输注的细胞量有关。

Objective： To investigate the effect of bone marrow mesenchymal stem cells （mMSC） infusion on the reconstruction of hematopoiesis function in mice with critical irradiation injury. Methods： Female C57BL/6 mice irradiated with 8 Gy ^60Co λ-rays were randomly divided into control group and mMSCs-treated group. Mice in the mMSCs treated group received mMSC of male C57BL/6 mice. The peripheral leukocyte, platelet, and erythrocyte counts, the pathological lesions, granulocyte-macrophage progenitors （CFU-GM）,and sex chromosome ratio were all examined. Results： All the animals in the control group （n= 6） died of hematopoiesis exhaustion 20 days after irradiation. The peripheral leukocyte of mMSC-treated group decreased greatly after bone marrow transplantation and quickly recovered 2 weeks later; till 28 days after transplantation,it recovered to 60% of the pre-irradiation level, mMSCs infusion promoted the quick recovery of the nucleated bone marrow cells and CFU-GM,improving the marrow morphology. The transplanted mMSC could be detected in the recipients 42 days after transplantation. Conclusion： Solo-injection of mMSCs can enhance hematopoietic reconstitution in mice with critical irradiation injury. The period of imbedding is related to the dose of infusion.