Survivin和Bcl-2调节槲皮素诱导的A549细胞凋亡

Survivin and Bcl-2 modulate apoptosis in A549 cell induced by quercetin

目的研究槲皮素诱导肺腺癌细胞A549凋亡,探讨survivin和Bcl-2在槲皮素诱导A549细胞凋亡中的调节作用。方法分别采用MTT法、荧光染色、流式细胞仪和免疫细胞化学观察了槲皮素对肺腺癌A549细胞增殖、凋亡、细胞周期和蛋白表达的影响。结果槲皮素抑制肺腺癌A549细胞增殖的作用明显,且呈浓度和时间依赖性。形态学检测显示出细胞凋亡的特征变化,槲皮素能使肺腺癌A549细胞周期阻滞于G0/G1期,且A549细胞survivin和Bcl-2蛋白表达同时下降,而caspase-3活性升高。结论槲皮素能诱导A549细胞凋亡,其机制可能是使肺腺癌A549细胞周期阻滞于G0/G1期,并同时下调survivin和Bcl-2蛋白的表达,直接激活caspase-3而诱导A549细胞凋亡。

Aim To investigate the apoptosis of lung cancer cells A549 induced by quercetin and the regulation of survivin and Bcl-2 on A549 cells induced by quercetin. Methods MTT, fluorescence stain, flow cytometric analysis and immunocytochemistry stain were carried inhibition on out. Results Quercetin had a significant growth and proliferation of A549 cell in a concentration-and time-dependent manner. Evidence was provided that apoptosis occurred in A549 cells treated with quercetin using fluorescence microscopy. Quercetin arrested A549 ceils at the G0/G1 phase by FCM analyses. Expression of survivin and Bcl-2 protein were decreased, and activity of caspase-3 were enhanced. Conclusion Quercetin could induce apoptosis of A549 cells. The arrested cell cycle and the down-regulation of survivin and Bcl-2 protein could activate caspase-3 resulting in cells apoptosis, which may contribute to the apoptosis mechanisms. The down-regulated survivin and Bcl-2 may play an important role in A549 cells apoptosis induced by quercetin.