摘要
Objective: To establish the method of real time fluorescence quantitative RT-PCR for detecting the expression of Survivin mRNA in nasopharyngeal carcinoma (NPC) tissues. Methods: The total RNA was extracted from NPC cell line CNE-2 and tissues with Trizol and then been transcribed reversely to cDNA, a method of real time fluorescence quantitative RT-PCR for detecting the expression of Survivin mRNA in NPC tissues had been established, in which chronic nasopharyngitis patients' nasopharynx tissues treated as control group. Results: The expression of Survivin mRNA all could be detected either in CNE-2 cells, NPC tissues or in chronic nasopharyngitis patients' nasopharynx tissues, and there was higher the expression level of Survivin mRNA in NPC tissues than which in chronic nasopharyngitis patients' nasopharynx tissues, the difference was significant (P < 0.01). The expression of Survivin mRNA could be detected both in stage I + II and stage III + IV NPC, and there was no significant difference in relative quantifications of gene expression between these two groups (P > 0.05). There was no relationship between Survivin mRNA expression and age and sex of NPC patients (P > 0.05). Conclusion: Real time fluorescence quantitative RT-PCR is a rapid, effective and high sensitive method for detecting the expression of Survivin mRNA in NPC tissues. The overexpression of Survivin mRNA may play some roles in pathogenesis of NPC.
Objective: To observe the expression of DNA-dependent protein kinase (DNA-PKcs) in the nasopharyngeal tissues during carcinogenesis. Methods: Patients including 30 cases of nasopharyngitis, 42 cases of nasopharyngeal atypical hyperplasia and 34 cases of nasopharyngeal carcinoma (NPC) were chosen from Zhongshan area in which there is high incidence rate of NPC. The expression of DNA-PKcs in the nasopharyngeal tissue was examined by immunohistochemistry. Results: The expression rates of DNA-PKcs in the nasopharyngitis tissue, the nasopharyngeal precancerous lesion and the NPC were 6.7%, 64.3% and 55.9%, respectively (P〈 0.001). Conclusion: The expression of DNA-dependent protein kinase may play an important role in nasopharyngeal tissue carcinogenesis.
基金
China Postdoctoral Foundation (No: 2005038496).
关键词
鼻咽炎
鼻咽癌前期损害
癌发生
临床研究
nasopharyngitis
nasopharyngeal precancerous lesion
nasopharyngeal carcinoma
DNA-dependent proteinkinase (DNA-PKcs)
clinical study