摘要
目的测定不同厂家丹参注射液中丹参素和原儿茶醛的含量,以考察其质量差异。方法RP—HPLC法。Kromasil ODS—C18色谱柱(250mm×4.6mm,5μm);流动相:甲醇:0.3%冰醋酸(22:78);流速1.0ml·min^-1;检测波长281nm。结果不同厂家丹参注射液中丹参素和原儿茶醛的含量分别为:0.4979~7.5885g·L^-1,0.1934~0.6358g·L^-1。结论不同厂家丹参注射液中丹参素和原儿茶醛的含量存在较大差异。
Aim To determine the contents of danshensu and protoeatechualdehyde in danshen injeetionrs from different manufactures. Methods RP-HPLC was applied for quantitative analysis of danshensu and protoeateehualdehyde. Kromasil ODS-C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-0.3% acetic acid (22:78 ). The flow rate was at 1.0 ml · min^-1 , and the diode array detector was set at 281 nm. Results The content of danshensu in danshen injeetions from different manufacturers was in the range of 0. 4979 -7. 5885 g · L^-1 , and the content of protocatechualdehyde was in the range of 0. 1934 -0. 6358 g · L^-1. Conclusion Significant difference was found in contents of danshensu and protoeateehualdehyde in danshen injections from different manufacturers.
出处
《安徽医药》
CAS
2008年第11期1055-1056,共2页
Anhui Medical and Pharmaceutical Journal