HPLC法测定不同厂家丹参注射液中丹参素和原儿茶醛的含量

Determination of danshensu and protocatechualdehyde in danshen injections from different manufacturers by HPLC

目的测定不同厂家丹参注射液中丹参素和原儿茶醛的含量，以考察其质量差异。方法RP—HPLC法。Kromasil ODS—C18色谱柱（250mm×4．6mm，5μm）；流动相：甲醇：0．3％冰醋酸（22：78）；流速1．0ml·min^-1；检测波长281nm。结果不同厂家丹参注射液中丹参素和原儿茶醛的含量分别为：0．4979～7．5885g·L^-1，0．1934～0．6358g·L^-1。结论不同厂家丹参注射液中丹参素和原儿茶醛的含量存在较大差异。

Aim To determine the contents of danshensu and protoeatechualdehyde in danshen injeetionrs from different manufactures. Methods RP-HPLC was applied for quantitative analysis of danshensu and protoeateehualdehyde. Kromasil ODS-C18 column （250 mm × 4.6 mm, 5 μm） was used. The mobile phase was methanol-0.3% acetic acid （22：78 ）. The flow rate was at 1.0 ml · min^-1 , and the diode array detector was set at 281 nm. Results The content of danshensu in danshen injeetions from different manufacturers was in the range of 0. 4979 -7. 5885 g · L^-1 , and the content of protocatechualdehyde was in the range of 0. 1934 -0. 6358 g · L^-1. Conclusion Significant difference was found in contents of danshensu and protoeateehualdehyde in danshen injections from different manufacturers.