cDNA芯片中重复检测值的处理方法对差异表达基因选择的影响

Effects of the treatments of the repeat measurements on the selection of differential expression genes in cDNA microarrays

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摘要采用六套cDNA数据,两种常用的方法处理表达谱中对应同一条Unigene的多条克隆的表达值,分析对利用SAM和T-test筛选差异基因的影响。结果显示:表达谱中对应同一条Unigene的多个克隆之间的相关性不高,并存在许多负相关;两种处理重复检测值的方法对差异表达基因的选择有很大的影响。 Using six cDNA data sets, we employed two commonly used methods to deal with the expression value of one Unigene corresponding to multiple measured clones and analyze the effects of the two methods on selecting DEGs by SAM and T- test. The results showed that the expression correlations of mutiple clones corresponding to one Unigene were not high and exhibited many negative values. Two different methods for dealing with the repeat measurements affect greatly the selection of differential expression genes in cDNA microarrays.

作者吕莹丽于梁梁郭政王栋杨强

机构地区哈尔滨医科大学生物信息学系

出处《生物信息学》 2008年第3期97-99,122,共4页 Chinese Journal of Bioinformatics

基金国家自然科学基金(30370388 30670539)

关键词差异表达基因基因表达谱相关 differential expression gene gene expression profile correlation

分类号 Q61 [生物学—生物物理学]

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1于梁梁,王栋,吕莹丽,肖会,杨强,王晨光,郭政,李霞.cDNA芯片重复探针检测值的一致性分析[J].高技术通讯,2009,19(1):95-98.

生物信息学

2008年第3期

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cDNA芯片中重复检测值的处理方法对差异表达基因选择的影响

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