细粒棘球绦虫——原头蚴在两种细胞培养液中体外培养的初步观察

In vitro cultivation protoscoleces of the protoslices of Echinococcus granulosus in medium RPMI-1640 and MEM

通过原头蚴在两种细胞培养液中（RPMI-1640和MEM）培养，观察其存活、生长及发育情况，从而为研究寄生虫发育提供最基本的数据资料。方法：细粒棘球绦虫幼虫——原头蚴培养的细胞培养基（RPMI-1640和MEM）分为4组：Ⅰ组为纯RPMI-1640培养液；Ⅱ组为含10％的小牛血清的1640培养液；Ⅲ组为纯MEM培养液；Ⅳ组为含10％的小牛血清的MEM培养液；并进行对比观察。结果：培养15d的原头蚴的成活率分别为：Ⅰ组69．87％、Ⅱ组80．35％、Ⅲ组50．25％、Ⅳ组60．32％；成囊率分别为：Ⅰ组80．58％、Ⅱ组90．25％、Ⅲ组35．65％、Ⅳ组45．89％；头节外翻率分别为：Ⅰ组95．50％、Ⅱ组98．65％、Ⅲ组60．52％、Ⅳ组70．98％。结论：大多数虫体在早期向囊发育，一部分虫体头节外翻，并伴有规律的伸缩运动，但随时间的延长虫体运动减缓，又向囊蚴发育。通过对细粒棘球绦虫的原头蚴的体外培养，初步表明含有10％小牛血清的细胞培养基RPMI-1640较适合原头蚴的生长发育，在普通培养箱中培养，最适温度在37—40℃，培养液pH=7．2，初步建立了原头蚴体外培养的方法。

To option cultural conditions for cultivation of protoscoleces of Echinococcus and to observe their growth and development granulosus in virto. Methods： Protoscoleces（PSC） were collected from hydatid cysts of E. granulosus and the PSC were cultured in two medias ,RPMI - 1640 and MEM with or without fetal calf serum （FCS）. The cultivation was observed using anormal optical miaroseopy. Rusults：viability rate of the PSC cultured for 15 days was 69.8% in RPMI - 1640 without FCS;80.4% in RPMI - 1640 with FCS ;50.3% in MEM without FCS and 60.3% in MEM with FCS. The rate of encystation was 80.58% in RPMI - 1640 without FCS; 90.25% in RPMI- 1640 with FCS ;35.65% in MEM without FCS and 45.89% in MEM with FCS . The rate extraversion of Scolex was 95.50% in RPMI- 1640 without FCS,98.65% in RPMI- 1640 with FCS ,60.52% in MEM without FCS and 70.98% in MEM with FCS . In the medium RPMI -1640 with FCS, 90.3% of PSC developed to hydatid cysts in two weeks of in vitro cultivation. Conclusion： The most PSC were developed to cyst formotion although in the early stage , some of the PSC developed to adult worm, but later changed to cysts. Medium RPMI - 1640 with FCS is more suitable medium for culturing PSC to hydatid cysts in vitro.