N-乙酰-L-半胱氨酸对大鼠胃缺血/再灌注损伤的影响

Eeffects of N-acetylcysteine on gastric ischemia/reperfusion injury in rats

目的研究抗氧化剂N-乙酰-L-半胱氨酸(N-acetylcysteine,NAC)对胃缺血/灌注(gastric ischemia/reperfusion,GI/R)损伤的影响。方法采用股静脉注射NAC后,夹闭大鼠腹腔动脉30 min,再灌注1 h的GI/R模型,计数胃黏膜损伤指数(gastric mucosal damage index,GMDI),测定胃黏膜组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,RT-PCR法检测凋亡相关因子环氧合酶-2(COX-2)的表达。结果股静脉注射NAC能降低GI/R胃黏膜损伤指数,使胃黏膜组织中MDA含量减少,SOD活性增强,凋亡相关因子COX-2的表达减少。结论NAC对大鼠GI/R损伤具有保护作用,这种保护作用是通过抑制氧化应激,减少胃黏膜细胞凋亡而实现的。

Objective To study the effect of antioxidant N-acetylcysteine（NAC） on gastric ischemia/reperfusion（GI/R） in rats.Methods After injection of NAC into femoral vein,the GI/R model was established by clamping the celiac artery for 30 min to induced ischemia and allowing reperfusion for 1 h.The rats were finally sacrificed to investigate gastric mucosal damage index（GMDI）,determine the content of malondialdehyde（MDA） and the activity of superoxide dismutase（SOD） in gastric mucosa.The expression of apoptosis-related COX-2 was detected by RT-PCR. Results Injection of NAC into femoral vein could decrease gastric mucosal damage index,reduce the content of MDA,raise the activity of SOD and inhibit the expression of COX-2 in gastric mucosa following GI/R.Conclusion NAC can protect against GI/R injury by inhibiting oxidative stress and reducing cell apoptosis in gastric mucosa.