肺癌患者外周血淋巴细胞免疫分型检测的临床意义

Clinical significance of changes in peripheral blood lymphocyte subsets in patients with lung cancer

目的探讨肺癌患者外周血淋巴细胞免疫分型,了解肺癌患者的基线免疫状态。方法采用流式细胞仪分别检测60例肺癌患者(肺癌组)和20例正常人(对照组)外周血淋巴细胞免疫分型,并对两组进行比较。结果肺癌患者CD3(+)、CD4(+)、自然杀伤细胞(NK细胞)比例及CD4(+)/CD8(+)比值均低于对照组(P<0.05),CD8(+)细胞比例高于对照组(P<0.05),CD19(+)细胞比例与对照组相比差异无显著性(P>0.05)。结论肺癌患者免疫功能与正常人群相比存在降低趋势,外周血淋巴细胞免疫分型可作为肺癌患者免疫监测的一种方法。

Objective To investigate the baseline immunological status in patients with lung cancer by analyzing the changes in peripheral blood lymphocyte subsets.Methods Lymphocyte subsets in the peripheral blood were detected by flow cytometry in 60 patients with lung cancer and 20 healthy controls.Results The lung cancer group had a lower percentages of CD3（＋）,CD4（＋） lymphocytes,NK cell,and lower CD4（＋）/CD8（＋） ratio than the control group（P〈0.05）,and had a higher percentage of CD8（＋） cells than the control group（P〈0.05）.There was no significant difference in the percentages of CD19（＋） cells between the lung cancer group and the control group（P〉0.05）.Conclusion The immunologic functions in patients with lung cancer were generally compromised in comparison with a healthy population.Detection of the variations of lymphocyte subsets in peripheral blood may be used in the monitoring of immune function for patients with lung cancer.