天麻多糖提取与自由基清除作用研究

Extraction and free radicals scavenging activities of polysaccharides from Gastrodia elata B1

目的:研究活性天麻多糖的提取与自由基清除作用。方法:以水提醇沉法制备天麻粗多糖,CTAB季铵盐络合法、凝胶过滤柱层析进一步纯化;利用Feton体系和邻苯三酚自氧化反应,采用比色法测定天麻多糖对OH·及O2-·自由基的清除作用。结果:通过正交实验获得天麻多糖提取工艺为料水比1∶40、浸提温度80℃、提取次数2次,75%乙醇沉淀;在相同的试验体系浓度下,天麻粗多糖及纯化多糖对OH·的IC50分别为1.18、1.62mg/mL;对O2-·的IC50分别为0.81、1.03mg/mL。结论:天麻多糖对氧自由基具有较佳清除能力。

Objective： To study the Gastrodia elata B1 polysaccharides（GPS） extraction and free radicals scavenging activities. Methods： The polysacchaddes was extracted from Gastrodia elata B1 by water and precipitated by ethanol, pudfied with CTAB and the sepharose 6-fast flow chromatography. In the vitro simulated chemical reaction system, the polysaccharide scavenging effect on OH. with Fenton reaction and O^2-. with Pyrogallol Autoxidation Method was studied. Results： The optimal extraction technology conditions were that the temperature was 80 ℃, the extraction time was twice, the solid-liquid ratio was 1：40 and the precipitating ethanol concentration was 75%. The respective IC50 of raw GPS and refined GPS to OH. was 1.18 mg/mL and 1.62 mg/mL, to O^2-. was 0.81 mg/mL and 1.03 mg/mL. Conclusion： These results clearly indicated that GPS had a good free radicals scavenging activity.