UASB生物制氢反应系统生物强化作用研究

Bioaugmentation Study on UASB Biohydrogen Production System

采用上流式厌氧污泥床（UASB）反应器，以糖蜜废水为底物，利用厌氧活性污泥发酵产氢．向反应器中投加高产氢微生物产酸克雷伯氏菌HP1。探讨了生物强化作用对反应器产氢能力的影响．研究表明：在污泥接种量为30．0gVSS／L、启动负荷为6．0kgCOD／（m^3·d）、水力停留时间（HRT）为9h、投菌量为3％的条件下对生物制氢系统进行强化。可使反应系统产氢能力提高25％，并形成丁酸型发酵产氢，液相末端发酵产物中丁酸和乙酸的含量占挥发酸总含量的63％以上，气相中氢气含量在40％～52％之间。最大产氢量达4．52L/d．

Bioaugmentation with hydrogen producing strain Klebsiella oxytoca HP1 in up-flow anaerobic sludge bed （UASB） reactor was studied. The optimum start-up condition for reactor was obtained as： sludge inoculants of 30.0 gVSS/L,initial organic loading rate of 6.0 kg COD/（m^3· d）, hydraulic retention time of 9 h, and bacteria dosing inoculants of 3 %. Under the optimal conditions,the hydrogen production increased by 25 %,the hydrogen content in the biogas was about 40%-52%, the maximal hydrogen rate was 4.52 L/d.