面粉中偶氮甲酰胺含量的高效液相色谱法测定

Determination of Azodicarbonamide in Flour by High-performance Liquid Chromatography

面粉中偶氮甲酰胺（azodicarbonamide，ADA）用丙酮提取，提取液氮吹浓缩后用20mmol／L乙酸铵的水溶液定容，并用正己烷脱脂。样液供高效液相色谱仪测定，外标法定量。方法的线性范围为0—50mg／L（r=1．000），定量下限为1．0mg／kg。在面粉基质中分别添加1．0、2．0、50．0mg／kg 3个水平的偶氮甲酰胺标准品，方法的回收率为80％～92％，相对标准偏差小于7％。

The azodicarbonamide （ADA）in flour was extracted with acetone. The extract was then concentrated under nitrogen and diluted to 1 mL with 20 mmol/L ammonium acetate aqueous solution. After removed the lipid by hexane, the extract was analyzed by HPLC with external standard method. The method has a good linearity in the range of 0 -50 mg/L（ r = 1. 000）with the quantification limit of 1.0 mg/kg. The recoveries of ADA from flour spiked at three levels of 1.0, 2.0 and 50.0 mg/kg were in the range of 80% - 92% with the relative standard deviations less than 7% .