反式白藜芦醇及反式白藜芦醇苷对大鼠肝微粒体蛋白质及细胞色素P450的影响

Effects of Trans-resveratrol and Trans-piceid on Content of Microsomal Protein,Cytochrome P450 of Rats

目的:研究反式白藜芦醇及反式白藜芦醇苷对大鼠肝质量、肝微粒体蛋白质及细胞色素P450的影响。方法:大鼠分为空白对照组、反式白藜芦醇组及反式白藜芦醇苷组,分别连续给予等摩尔量的反式白藜芦醇、反式白藜芦醇苷(116.69、200 mg/(kg.d)),连续灌胃给药7 d后,分光光度法测定大鼠肝质量、肝微粒体蛋白质浓度及细胞色素P450质量分数,氨基比林-N-脱甲基酶、红霉素脱甲基酶和7-乙氧基-3-异吩哐恶唑酮脱烃酶的活性。结果:反式白藜芦醇组及反式白藜芦醇苷组大鼠肝质量、肝微粒体蛋白浓度与空白对照组比无统计学意义;而2个剂量组大鼠肝微粒体中细胞色素P450、氨基比林-N-脱甲基酶、红霉素-N-脱甲基酶和7-乙氧基-3-异吩哐恶唑酮脱烃酶的活性与空白对照组比有统计学意义(P<0.05),对细胞色素P450酶有抑制作用,但剂量组间无统计学意义。结论:反式白藜芦醇与反式白藜芦醇苷可引起肝药酶对某些药物代谢的改变。

Objective： To study the effects of tmns-resveratrol and trans-piceid on liver weight, contents of microsomal protein and cytechrome P450. Methods： Rats were divided into trans-resveratrol, trans-piceid and control groups. The UV method was used to detect the liver weight, contents of microsomal protein and cytochrome P450, the activities of aminopyrine-N-demethylase, erythromycin-N-demethylase and coumarin 7-hydmxylation of rats, after administration with equimolar trans-resveratrol（116.69 mg/（kg·d））and trans-piceid（200 mg/（kg·d））for 7 days. Results： The study showed no significant differences on liver weight and contents of microsomal protein among three groups. Compared with control group, the activities of cytochrome P450, aminopyrine-N-demethylase, erythromycin-N-demethylase and coumarin 7-hydroxylation between two dosage groups（ P 〈 0.05）had statistical significance. But there was no significant difference between two dosage groups. Conclusion： Trans-resveratrol and trans-piceid can result in alteration of metabolism of some drugs affected by drug metabolizing enzyme of liver.