摘要
从乌克兰引进的七叶树种子中取材作为外植体,采用不同的消毒剂及在MS培养基中添加不同浓度的赤霉素(GA3)、细胞分裂素(6-BA)对七叶树离体培养植株生长影响的研究结果表明:外植体消毒以50%的氯胺水溶液处理10m in效果最好;MS培养基中不需要添加GA3,而添加浓度1.5μg/L 6-BA的MS培养基中,幼苗能更好的生长并能获得大量的具有典型性状的植株。
Take the seed of Aesculus came was introduced from Ukraine as explants. The effect of different disinfectants, MS medium that add in different concentrations of gibberellin （GA3 ） and cytokinin （6 - BA） on the plants growth were studied. The results showed that the seedling can growth better and access to a large number of plant in a typical characters on the following conditions ： （ 1 ） The explants disinfected with 50% of the chloramine solution for 10min. （2） MS medium without the addition of GA3. （3） MS medium added in 6 - BA of 1. 5 μg/L.
出处
《林业科技》
北大核心
2008年第5期1-3,共3页
Forestry Science & Technology
基金
浙江省科技厅重点科研国际合作项目(2005C24012)
关键词
七叶树
离体培养
氯胺
GA3
6-BA
Aesculus came
Culture in vitro
Chloramines
GA3 , 6 - BA
